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    Structured Review

    Illumina Inc buffer large kit illumina
    Buffer Large Kit Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+large+kit+illumina/Illumina+Tagment+DNA+Enzyme+and+Buffer+Large+Kit/pm41916318-278-166-169
    Average 97 stars, based on 399 article reviews
    buffer large kit illumina - by Bioz Stars, 2026-09
    97/100 stars

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    Article Title: Targeting the mSWI/SNF complex in POU2F-POU2AF transcription factor-driven malignancies.
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    Article Title: Genomic copy-number variants drive apoptotic evasion underlying acquired resistance to immune checkpoint inhibitors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sodium butyrate Thermo Fisher Scientific Cat# A11079.36 HEPES Thermo Fisher Scientific Cat# 15630080 Recombinant human IL-2 Miltenyi Biotec Cat# 130-097-748 DynabeadsTM Human T-Activator CD3/ CD28 for T Cell Expansion and Activation Gibco Cat# 11161D RetroNectin Takara Cat# T110A Cryostor CS10 cryopreservation medium Biolife Solutions Cat# 210102 G418 disulfate salt MilliporeSigma Cat# A1720-1G Blasticidin S HCl Gibco Cat# R21001 Puromycin MilliporeSigma Cat# P8833-25MG Hygromycin B Thermo Fisher Scientific Cat# AAJ6068103 Polybrene Infection / Transfection Reagent MilliporeSigma Cat# TR-1003-G RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89901 HaltTM Phosphatase Inhibitor Single-Use Cocktail Thermo Fisher Scientific Cat# 78428 PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23227 Tween 80 Thermo Fisher Scientific Cat# BP338-500 4% Paraformaldehyde in PBS Thermo Fisher Scientific Cat# J61899.AP Crystal violet solution MilliporeSigma Cat# V5256 Tris-HCl (PH7.4) MilliporeSigma Cat# T2194 Tween 20 Andwin Scientific Cat# NC9022994 Nonidet P40 Substitute MilliporeSigma Cat# 74385 NaCl Invitrogen Cat# AM9760G MgCl2 Invitrogen Cat# AM9530G Triton X-100 Thermo Fisher Scientific Cat# ICN19485483 QIAGEN Protease (30 AU) Qiagen Cat# 19157 0.5M EDTA Thermo Fisher Scientific Cat# R1021 Xylene Fisher Scientific Cat# X5-1 Ethyl Alcohol Sigma-Aldrich Cat# E7023 Critical commercial assays QIAGEN AllPrep DNA/RNA Mini Kit Qiagen Cat# 80204 QIAGEN QIAamp DNA FFPE Tissue Kit Qiagen Cat# 56404 QIAGEN FlexiGene DNA Kit Qiagen Cat# 51206 Pan T Cell Isolation Kit Miltenyi Biotec Cat# 130-096-535 Tumor Dissociation Kit, mouse Miltenyi Biotec Cat# 130-096-730 FFPE Tissue Dissociation Kit Miltenyi Biotec Cat# 130-118-052 Illumina Tagment DNA Enzyme and Buffer Large Kit Illumina Cat# 20034198 KAPA HotStart PCR Kit, with dNTPs Roche Cat# KK2502 Qubit dsDNA HS (High Sensitivity) Assay Kit Invitrogen Cat# Q32851 Qubit dsDNA BR (Broad-Range) Assay Kit Invitrogen Cat# Q32850 Deposited data WES This paper European Genome-phenome Archive (EGA): EGAS50000001055 WGS This paper EGA: EGAS50000001055 WES Zaretsky et al. 15 SRA: SRP076315 WES Hugo et al. 11 SRA: SRP067938, SRP090294 WES Liu et al., 13 Hugo et al., 44 Moriceau et al., 45 Shi et al. 46 SRA: SRP049746 Copy number profiles of TCGA-SKCM cohort TCGA, PanCancer Atlas https://www.cbioportal.org/ (Continued on next page) e2 Immunity 58, 1–14.e1–e9, November 11, 2025

    Article Title: Foxp3 and BATF cooperatively direct cis-regulatory programs and gene expression for effector Treg cell differentiation.
    Article Snippet: Article Foxp3 and BATF cooperatively direct cis-regulatory programs and gene expression for effector Treg cell differentiation

    Transfection:

    Article Title: CRISPR activation screens identify the SWI/SNF ATPases as suppressors of ferroptosis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BRM Cell Signaling Cat#11966; RRID: AB_2797783 BRG1 D1Q7F Cell Signaling Cat#49360; RRID:AB_2728743 Beta-Tubulin Cell Signaling Cat#2128; RRID:AB_823664 NRF2 (EP1808Y) Abcam Cat#ab62352; RRID:AB_944418 H3K27me3 Thermofisher Cat#MA511198; RRID:AB_11000749 H3 Cell Signaling Cat#9715; RRID:AB_331563 KEAP1 (P586) Cell Signaling Cat#4678; RRID:AB_10548196 TKT (E7O4M) Cell Signaling Cat#64414; RRID:AB_3097751 GCLC Cell Signaling Cat#48005; RRID: AB_3101780 AKR1B10 (EPR14421) Abcam Cat#ab192865, RRID: AB_3101779 POPDC3 Novus Biologicals Cat#NBP159476; RRID:AB_11034825 BRM for Cut&Run Epicypher Cat#13-2006; RRID:AB_3101778 NRF2 for Cut&Run Active Motif RRID:AB_2793695 SMARCC1 Cell Signaling Cat#11956; RRID:AB_2797776 H3K4me1 Epicypher Cat#13-0057; RRID:AB_3076424 H3K4me3 for Cut&Run Epicypher Cat#13-0041; RRID:AB_3076423 H3K27ac Cell Signaling Cat#8173; RRID:AB_10949503 IgG Epicypher Cat#13-0042; RRID:AB_2923178 Chemicals, Peptides, and Recombinant Proteins Tazemetostat SelleckChem Cat#S7128 Nec1s SelleckChem Cat#S8641 Z-VAD-FMK SelleckChem Cat#S7023 Ferrostatin-1 SelleckChem Cat#S7243 ML210 MIllipore Sigma SML0521 RSL3 Millipore Sigma SML2234 BRM-014 Genentech N/A BRM degrader A947 Genentech N/A Inactive Degrader Control Genentech N/A Imidazole Ketone Erastin SelleckChem Cat#S8877 FIN56 Genentech N/A Critical Commercial Assays Human whole genome SAM CRISPRa pooled lentiviral libraries Sigma Cat#HSAMZEO-1KT TRC3 whole genome lentiviral ORF pool Sigma Cat#ORFPOOLWG – 1SET Chromium Single Cell Gene Expression Reagent Kit v3.1 10x genomics Cat#1000121 Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kit 10x genomics Cat#1000285 Image-IT Lipid peroxidation kit Thermofisher Cat#C10445 Hoescht Thermofisher Cat#33342 CellBrite Steady 650 Biotium Cat#30108 NEBNext 23 PCR Master mix NEB Cat#M0541L Q5 2X Hot start PCR master mix NEB Cat#M0494L QIAquick PCR CleanUp Kit Qiagen Cat#28104 (Continued on next page) Cell Reports 43, 114345, June 25, 2024 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Qiagen MinElute Cleanup Kit Qiagen Cat#28206 Lipofectamine RNAiMAX Transfection Reagent ThermoFisher Cat#13778150 RNeasy extraction kit Qiagen Cat#74034 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7573 Cyquant Direct reagent ThermoFisher Cat#C35011 CUTANA ChIC/CUT&RUN Kit Epicypher Cat#14-1048 CUTANA CUT&RUN Library Prep Kit Epicypher Cat#14-1001 Tagment DNA Enzyme and Buffer Large Kit Illumina Cat20034197 High Sensitivity D1000 Screen Tape and Reagents Agilent Cat#5067-5587 LysoTrackerTM Green DND-26 ThermoFisher Cat#L7526 High Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368813 Taqman Assay for p53 Applied Biosystems Mm01731290_g1 Taqman Assay for SMARCA4 Applied Biosystems Mm01151944_m1 Taqman assay for TUBG Applied Biosystems Mm00506159_m1 Deposited Data All sequencing data Super Series GEO GEO: GSE245249 Cut&Run GEO GEO: GSE245237, GEO: GSE266343,GEO: GSE266350 ATAC-seq GEO GEO: GSE245238, GEO: GSE245246. .. GEO: GSE245247, GEO: GSE245248 RNA-seq GEO GEO: GSE245239, GEO: GSE245245 scRNA-seq GEO GEO: GSE245241 snRNA-seq GEO GEO: GSE245242 scATAC-seq GEO GEO: GSE245243 Amplicon-seq GEO GEO: GSE245244 Experimental Models: Cell Lines KP4, sex: male ATCC RRID: CVCL_1338 PANC-1, sex: male ATCC CRL-1469 MiaPaCa-2, sex: male ATCC CRL-1420 NCI H460, sex: male ATCC HTB-177 NCI H1693, sex: female ATCC CRL-5887 NCI H1793, sex: female ATCC CRL-5896 A549, sex: male ATCC CCL-185 H446, sex: male ATCC HTB-171 H2023, sex: male ATCC CRL-5912 H1568, sex: female ATCC CRL-5876 H661, sex: male ATCC HTB-183 H838, sex: male ATCC CRL-5844 H2286, sex: female ATCC CRL-5938 KP4- inducible BRM oe cells This manuscript N/A KP4 cells overexpressing BRM or BRG1 This manuscript N/A Male mouse lung ATII cells derived organoids with BRG1 knockout This manuscript N/A PANC1 cells overexpressing BRM or BRG1 This manuscript N/A MiaPaCa2 cells overexpressing BRM or BRG1 This manuscript N/A BRM/BRG1 oe cells with NRF2 knock out This manuscript N/A Oligonucleotides On Target Plus siNT pooled siRNA Dharmacon Cat#D-001810-10 On Target Plus siNRF2: pooled siRNA Dharmacon Cat#L-003755-00 (Continued on next page) 20 Cell Reports 43, 114345, June 25, 2024

    Extraction:

    Article Title: CRISPR activation screens identify the SWI/SNF ATPases as suppressors of ferroptosis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BRM Cell Signaling Cat#11966; RRID: AB_2797783 BRG1 D1Q7F Cell Signaling Cat#49360; RRID:AB_2728743 Beta-Tubulin Cell Signaling Cat#2128; RRID:AB_823664 NRF2 (EP1808Y) Abcam Cat#ab62352; RRID:AB_944418 H3K27me3 Thermofisher Cat#MA511198; RRID:AB_11000749 H3 Cell Signaling Cat#9715; RRID:AB_331563 KEAP1 (P586) Cell Signaling Cat#4678; RRID:AB_10548196 TKT (E7O4M) Cell Signaling Cat#64414; RRID:AB_3097751 GCLC Cell Signaling Cat#48005; RRID: AB_3101780 AKR1B10 (EPR14421) Abcam Cat#ab192865, RRID: AB_3101779 POPDC3 Novus Biologicals Cat#NBP159476; RRID:AB_11034825 BRM for Cut&Run Epicypher Cat#13-2006; RRID:AB_3101778 NRF2 for Cut&Run Active Motif RRID:AB_2793695 SMARCC1 Cell Signaling Cat#11956; RRID:AB_2797776 H3K4me1 Epicypher Cat#13-0057; RRID:AB_3076424 H3K4me3 for Cut&Run Epicypher Cat#13-0041; RRID:AB_3076423 H3K27ac Cell Signaling Cat#8173; RRID:AB_10949503 IgG Epicypher Cat#13-0042; RRID:AB_2923178 Chemicals, Peptides, and Recombinant Proteins Tazemetostat SelleckChem Cat#S7128 Nec1s SelleckChem Cat#S8641 Z-VAD-FMK SelleckChem Cat#S7023 Ferrostatin-1 SelleckChem Cat#S7243 ML210 MIllipore Sigma SML0521 RSL3 Millipore Sigma SML2234 BRM-014 Genentech N/A BRM degrader A947 Genentech N/A Inactive Degrader Control Genentech N/A Imidazole Ketone Erastin SelleckChem Cat#S8877 FIN56 Genentech N/A Critical Commercial Assays Human whole genome SAM CRISPRa pooled lentiviral libraries Sigma Cat#HSAMZEO-1KT TRC3 whole genome lentiviral ORF pool Sigma Cat#ORFPOOLWG – 1SET Chromium Single Cell Gene Expression Reagent Kit v3.1 10x genomics Cat#1000121 Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kit 10x genomics Cat#1000285 Image-IT Lipid peroxidation kit Thermofisher Cat#C10445 Hoescht Thermofisher Cat#33342 CellBrite Steady 650 Biotium Cat#30108 NEBNext 23 PCR Master mix NEB Cat#M0541L Q5 2X Hot start PCR master mix NEB Cat#M0494L QIAquick PCR CleanUp Kit Qiagen Cat#28104 (Continued on next page) Cell Reports 43, 114345, June 25, 2024 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Qiagen MinElute Cleanup Kit Qiagen Cat#28206 Lipofectamine RNAiMAX Transfection Reagent ThermoFisher Cat#13778150 RNeasy extraction kit Qiagen Cat#74034 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7573 Cyquant Direct reagent ThermoFisher Cat#C35011 CUTANA ChIC/CUT&RUN Kit Epicypher Cat#14-1048 CUTANA CUT&RUN Library Prep Kit Epicypher Cat#14-1001 Tagment DNA Enzyme and Buffer Large Kit Illumina Cat20034197 High Sensitivity D1000 Screen Tape and Reagents Agilent Cat#5067-5587 LysoTrackerTM Green DND-26 ThermoFisher Cat#L7526 High Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368813 Taqman Assay for p53 Applied Biosystems Mm01731290_g1 Taqman Assay for SMARCA4 Applied Biosystems Mm01151944_m1 Taqman assay for TUBG Applied Biosystems Mm00506159_m1 Deposited Data All sequencing data Super Series GEO GEO: GSE245249 Cut&Run GEO GEO: GSE245237, GEO: GSE266343,GEO: GSE266350 ATAC-seq GEO GEO: GSE245238, GEO: GSE245246. .. GEO: GSE245247, GEO: GSE245248 RNA-seq GEO GEO: GSE245239, GEO: GSE245245 scRNA-seq GEO GEO: GSE245241 snRNA-seq GEO GEO: GSE245242 scATAC-seq GEO GEO: GSE245243 Amplicon-seq GEO GEO: GSE245244 Experimental Models: Cell Lines KP4, sex: male ATCC RRID: CVCL_1338 PANC-1, sex: male ATCC CRL-1469 MiaPaCa-2, sex: male ATCC CRL-1420 NCI H460, sex: male ATCC HTB-177 NCI H1693, sex: female ATCC CRL-5887 NCI H1793, sex: female ATCC CRL-5896 A549, sex: male ATCC CCL-185 H446, sex: male ATCC HTB-171 H2023, sex: male ATCC CRL-5912 H1568, sex: female ATCC CRL-5876 H661, sex: male ATCC HTB-183 H838, sex: male ATCC CRL-5844 H2286, sex: female ATCC CRL-5938 KP4- inducible BRM oe cells This manuscript N/A KP4 cells overexpressing BRM or BRG1 This manuscript N/A Male mouse lung ATII cells derived organoids with BRG1 knockout This manuscript N/A PANC1 cells overexpressing BRM or BRG1 This manuscript N/A MiaPaCa2 cells overexpressing BRM or BRG1 This manuscript N/A BRM/BRG1 oe cells with NRF2 knock out This manuscript N/A Oligonucleotides On Target Plus siNT pooled siRNA Dharmacon Cat#D-001810-10 On Target Plus siNRF2: pooled siRNA Dharmacon Cat#L-003755-00 (Continued on next page) 20 Cell Reports 43, 114345, June 25, 2024

    Cell Viability Assay:

    Article Title: CRISPR activation screens identify the SWI/SNF ATPases as suppressors of ferroptosis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BRM Cell Signaling Cat#11966; RRID: AB_2797783 BRG1 D1Q7F Cell Signaling Cat#49360; RRID:AB_2728743 Beta-Tubulin Cell Signaling Cat#2128; RRID:AB_823664 NRF2 (EP1808Y) Abcam Cat#ab62352; RRID:AB_944418 H3K27me3 Thermofisher Cat#MA511198; RRID:AB_11000749 H3 Cell Signaling Cat#9715; RRID:AB_331563 KEAP1 (P586) Cell Signaling Cat#4678; RRID:AB_10548196 TKT (E7O4M) Cell Signaling Cat#64414; RRID:AB_3097751 GCLC Cell Signaling Cat#48005; RRID: AB_3101780 AKR1B10 (EPR14421) Abcam Cat#ab192865, RRID: AB_3101779 POPDC3 Novus Biologicals Cat#NBP159476; RRID:AB_11034825 BRM for Cut&Run Epicypher Cat#13-2006; RRID:AB_3101778 NRF2 for Cut&Run Active Motif RRID:AB_2793695 SMARCC1 Cell Signaling Cat#11956; RRID:AB_2797776 H3K4me1 Epicypher Cat#13-0057; RRID:AB_3076424 H3K4me3 for Cut&Run Epicypher Cat#13-0041; RRID:AB_3076423 H3K27ac Cell Signaling Cat#8173; RRID:AB_10949503 IgG Epicypher Cat#13-0042; RRID:AB_2923178 Chemicals, Peptides, and Recombinant Proteins Tazemetostat SelleckChem Cat#S7128 Nec1s SelleckChem Cat#S8641 Z-VAD-FMK SelleckChem Cat#S7023 Ferrostatin-1 SelleckChem Cat#S7243 ML210 MIllipore Sigma SML0521 RSL3 Millipore Sigma SML2234 BRM-014 Genentech N/A BRM degrader A947 Genentech N/A Inactive Degrader Control Genentech N/A Imidazole Ketone Erastin SelleckChem Cat#S8877 FIN56 Genentech N/A Critical Commercial Assays Human whole genome SAM CRISPRa pooled lentiviral libraries Sigma Cat#HSAMZEO-1KT TRC3 whole genome lentiviral ORF pool Sigma Cat#ORFPOOLWG – 1SET Chromium Single Cell Gene Expression Reagent Kit v3.1 10x genomics Cat#1000121 Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kit 10x genomics Cat#1000285 Image-IT Lipid peroxidation kit Thermofisher Cat#C10445 Hoescht Thermofisher Cat#33342 CellBrite Steady 650 Biotium Cat#30108 NEBNext 23 PCR Master mix NEB Cat#M0541L Q5 2X Hot start PCR master mix NEB Cat#M0494L QIAquick PCR CleanUp Kit Qiagen Cat#28104 (Continued on next page) Cell Reports 43, 114345, June 25, 2024 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Qiagen MinElute Cleanup Kit Qiagen Cat#28206 Lipofectamine RNAiMAX Transfection Reagent ThermoFisher Cat#13778150 RNeasy extraction kit Qiagen Cat#74034 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7573 Cyquant Direct reagent ThermoFisher Cat#C35011 CUTANA ChIC/CUT&RUN Kit Epicypher Cat#14-1048 CUTANA CUT&RUN Library Prep Kit Epicypher Cat#14-1001 Tagment DNA Enzyme and Buffer Large Kit Illumina Cat20034197 High Sensitivity D1000 Screen Tape and Reagents Agilent Cat#5067-5587 LysoTrackerTM Green DND-26 ThermoFisher Cat#L7526 High Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368813 Taqman Assay for p53 Applied Biosystems Mm01731290_g1 Taqman Assay for SMARCA4 Applied Biosystems Mm01151944_m1 Taqman assay for TUBG Applied Biosystems Mm00506159_m1 Deposited Data All sequencing data Super Series GEO GEO: GSE245249 Cut&Run GEO GEO: GSE245237, GEO: GSE266343,GEO: GSE266350 ATAC-seq GEO GEO: GSE245238, GEO: GSE245246. .. GEO: GSE245247, GEO: GSE245248 RNA-seq GEO GEO: GSE245239, GEO: GSE245245 scRNA-seq GEO GEO: GSE245241 snRNA-seq GEO GEO: GSE245242 scATAC-seq GEO GEO: GSE245243 Amplicon-seq GEO GEO: GSE245244 Experimental Models: Cell Lines KP4, sex: male ATCC RRID: CVCL_1338 PANC-1, sex: male ATCC CRL-1469 MiaPaCa-2, sex: male ATCC CRL-1420 NCI H460, sex: male ATCC HTB-177 NCI H1693, sex: female ATCC CRL-5887 NCI H1793, sex: female ATCC CRL-5896 A549, sex: male ATCC CCL-185 H446, sex: male ATCC HTB-171 H2023, sex: male ATCC CRL-5912 H1568, sex: female ATCC CRL-5876 H661, sex: male ATCC HTB-183 H838, sex: male ATCC CRL-5844 H2286, sex: female ATCC CRL-5938 KP4- inducible BRM oe cells This manuscript N/A KP4 cells overexpressing BRM or BRG1 This manuscript N/A Male mouse lung ATII cells derived organoids with BRG1 knockout This manuscript N/A PANC1 cells overexpressing BRM or BRG1 This manuscript N/A MiaPaCa2 cells overexpressing BRM or BRG1 This manuscript N/A BRM/BRG1 oe cells with NRF2 knock out This manuscript N/A Oligonucleotides On Target Plus siNT pooled siRNA Dharmacon Cat#D-001810-10 On Target Plus siNRF2: pooled siRNA Dharmacon Cat#L-003755-00 (Continued on next page) 20 Cell Reports 43, 114345, June 25, 2024

    CyQUANT Assay:

    Article Title: CRISPR activation screens identify the SWI/SNF ATPases as suppressors of ferroptosis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BRM Cell Signaling Cat#11966; RRID: AB_2797783 BRG1 D1Q7F Cell Signaling Cat#49360; RRID:AB_2728743 Beta-Tubulin Cell Signaling Cat#2128; RRID:AB_823664 NRF2 (EP1808Y) Abcam Cat#ab62352; RRID:AB_944418 H3K27me3 Thermofisher Cat#MA511198; RRID:AB_11000749 H3 Cell Signaling Cat#9715; RRID:AB_331563 KEAP1 (P586) Cell Signaling Cat#4678; RRID:AB_10548196 TKT (E7O4M) Cell Signaling Cat#64414; RRID:AB_3097751 GCLC Cell Signaling Cat#48005; RRID: AB_3101780 AKR1B10 (EPR14421) Abcam Cat#ab192865, RRID: AB_3101779 POPDC3 Novus Biologicals Cat#NBP159476; RRID:AB_11034825 BRM for Cut&Run Epicypher Cat#13-2006; RRID:AB_3101778 NRF2 for Cut&Run Active Motif RRID:AB_2793695 SMARCC1 Cell Signaling Cat#11956; RRID:AB_2797776 H3K4me1 Epicypher Cat#13-0057; RRID:AB_3076424 H3K4me3 for Cut&Run Epicypher Cat#13-0041; RRID:AB_3076423 H3K27ac Cell Signaling Cat#8173; RRID:AB_10949503 IgG Epicypher Cat#13-0042; RRID:AB_2923178 Chemicals, Peptides, and Recombinant Proteins Tazemetostat SelleckChem Cat#S7128 Nec1s SelleckChem Cat#S8641 Z-VAD-FMK SelleckChem Cat#S7023 Ferrostatin-1 SelleckChem Cat#S7243 ML210 MIllipore Sigma SML0521 RSL3 Millipore Sigma SML2234 BRM-014 Genentech N/A BRM degrader A947 Genentech N/A Inactive Degrader Control Genentech N/A Imidazole Ketone Erastin SelleckChem Cat#S8877 FIN56 Genentech N/A Critical Commercial Assays Human whole genome SAM CRISPRa pooled lentiviral libraries Sigma Cat#HSAMZEO-1KT TRC3 whole genome lentiviral ORF pool Sigma Cat#ORFPOOLWG – 1SET Chromium Single Cell Gene Expression Reagent Kit v3.1 10x genomics Cat#1000121 Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kit 10x genomics Cat#1000285 Image-IT Lipid peroxidation kit Thermofisher Cat#C10445 Hoescht Thermofisher Cat#33342 CellBrite Steady 650 Biotium Cat#30108 NEBNext 23 PCR Master mix NEB Cat#M0541L Q5 2X Hot start PCR master mix NEB Cat#M0494L QIAquick PCR CleanUp Kit Qiagen Cat#28104 (Continued on next page) Cell Reports 43, 114345, June 25, 2024 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Qiagen MinElute Cleanup Kit Qiagen Cat#28206 Lipofectamine RNAiMAX Transfection Reagent ThermoFisher Cat#13778150 RNeasy extraction kit Qiagen Cat#74034 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7573 Cyquant Direct reagent ThermoFisher Cat#C35011 CUTANA ChIC/CUT&RUN Kit Epicypher Cat#14-1048 CUTANA CUT&RUN Library Prep Kit Epicypher Cat#14-1001 Tagment DNA Enzyme and Buffer Large Kit Illumina Cat20034197 High Sensitivity D1000 Screen Tape and Reagents Agilent Cat#5067-5587 LysoTrackerTM Green DND-26 ThermoFisher Cat#L7526 High Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368813 Taqman Assay for p53 Applied Biosystems Mm01731290_g1 Taqman Assay for SMARCA4 Applied Biosystems Mm01151944_m1 Taqman assay for TUBG Applied Biosystems Mm00506159_m1 Deposited Data All sequencing data Super Series GEO GEO: GSE245249 Cut&Run GEO GEO: GSE245237, GEO: GSE266343,GEO: GSE266350 ATAC-seq GEO GEO: GSE245238, GEO: GSE245246. .. GEO: GSE245247, GEO: GSE245248 RNA-seq GEO GEO: GSE245239, GEO: GSE245245 scRNA-seq GEO GEO: GSE245241 snRNA-seq GEO GEO: GSE245242 scATAC-seq GEO GEO: GSE245243 Amplicon-seq GEO GEO: GSE245244 Experimental Models: Cell Lines KP4, sex: male ATCC RRID: CVCL_1338 PANC-1, sex: male ATCC CRL-1469 MiaPaCa-2, sex: male ATCC CRL-1420 NCI H460, sex: male ATCC HTB-177 NCI H1693, sex: female ATCC CRL-5887 NCI H1793, sex: female ATCC CRL-5896 A549, sex: male ATCC CCL-185 H446, sex: male ATCC HTB-171 H2023, sex: male ATCC CRL-5912 H1568, sex: female ATCC CRL-5876 H661, sex: male ATCC HTB-183 H838, sex: male ATCC CRL-5844 H2286, sex: female ATCC CRL-5938 KP4- inducible BRM oe cells This manuscript N/A KP4 cells overexpressing BRM or BRG1 This manuscript N/A Male mouse lung ATII cells derived organoids with BRG1 knockout This manuscript N/A PANC1 cells overexpressing BRM or BRG1 This manuscript N/A MiaPaCa2 cells overexpressing BRM or BRG1 This manuscript N/A BRM/BRG1 oe cells with NRF2 knock out This manuscript N/A Oligonucleotides On Target Plus siNT pooled siRNA Dharmacon Cat#D-001810-10 On Target Plus siNRF2: pooled siRNA Dharmacon Cat#L-003755-00 (Continued on next page) 20 Cell Reports 43, 114345, June 25, 2024

    Reverse Transcription:

    Article Title: CRISPR activation screens identify the SWI/SNF ATPases as suppressors of ferroptosis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BRM Cell Signaling Cat#11966; RRID: AB_2797783 BRG1 D1Q7F Cell Signaling Cat#49360; RRID:AB_2728743 Beta-Tubulin Cell Signaling Cat#2128; RRID:AB_823664 NRF2 (EP1808Y) Abcam Cat#ab62352; RRID:AB_944418 H3K27me3 Thermofisher Cat#MA511198; RRID:AB_11000749 H3 Cell Signaling Cat#9715; RRID:AB_331563 KEAP1 (P586) Cell Signaling Cat#4678; RRID:AB_10548196 TKT (E7O4M) Cell Signaling Cat#64414; RRID:AB_3097751 GCLC Cell Signaling Cat#48005; RRID: AB_3101780 AKR1B10 (EPR14421) Abcam Cat#ab192865, RRID: AB_3101779 POPDC3 Novus Biologicals Cat#NBP159476; RRID:AB_11034825 BRM for Cut&Run Epicypher Cat#13-2006; RRID:AB_3101778 NRF2 for Cut&Run Active Motif RRID:AB_2793695 SMARCC1 Cell Signaling Cat#11956; RRID:AB_2797776 H3K4me1 Epicypher Cat#13-0057; RRID:AB_3076424 H3K4me3 for Cut&Run Epicypher Cat#13-0041; RRID:AB_3076423 H3K27ac Cell Signaling Cat#8173; RRID:AB_10949503 IgG Epicypher Cat#13-0042; RRID:AB_2923178 Chemicals, Peptides, and Recombinant Proteins Tazemetostat SelleckChem Cat#S7128 Nec1s SelleckChem Cat#S8641 Z-VAD-FMK SelleckChem Cat#S7023 Ferrostatin-1 SelleckChem Cat#S7243 ML210 MIllipore Sigma SML0521 RSL3 Millipore Sigma SML2234 BRM-014 Genentech N/A BRM degrader A947 Genentech N/A Inactive Degrader Control Genentech N/A Imidazole Ketone Erastin SelleckChem Cat#S8877 FIN56 Genentech N/A Critical Commercial Assays Human whole genome SAM CRISPRa pooled lentiviral libraries Sigma Cat#HSAMZEO-1KT TRC3 whole genome lentiviral ORF pool Sigma Cat#ORFPOOLWG – 1SET Chromium Single Cell Gene Expression Reagent Kit v3.1 10x genomics Cat#1000121 Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kit 10x genomics Cat#1000285 Image-IT Lipid peroxidation kit Thermofisher Cat#C10445 Hoescht Thermofisher Cat#33342 CellBrite Steady 650 Biotium Cat#30108 NEBNext 23 PCR Master mix NEB Cat#M0541L Q5 2X Hot start PCR master mix NEB Cat#M0494L QIAquick PCR CleanUp Kit Qiagen Cat#28104 (Continued on next page) Cell Reports 43, 114345, June 25, 2024 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Qiagen MinElute Cleanup Kit Qiagen Cat#28206 Lipofectamine RNAiMAX Transfection Reagent ThermoFisher Cat#13778150 RNeasy extraction kit Qiagen Cat#74034 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7573 Cyquant Direct reagent ThermoFisher Cat#C35011 CUTANA ChIC/CUT&RUN Kit Epicypher Cat#14-1048 CUTANA CUT&RUN Library Prep Kit Epicypher Cat#14-1001 Tagment DNA Enzyme and Buffer Large Kit Illumina Cat20034197 High Sensitivity D1000 Screen Tape and Reagents Agilent Cat#5067-5587 LysoTrackerTM Green DND-26 ThermoFisher Cat#L7526 High Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368813 Taqman Assay for p53 Applied Biosystems Mm01731290_g1 Taqman Assay for SMARCA4 Applied Biosystems Mm01151944_m1 Taqman assay for TUBG Applied Biosystems Mm00506159_m1 Deposited Data All sequencing data Super Series GEO GEO: GSE245249 Cut&Run GEO GEO: GSE245237, GEO: GSE266343,GEO: GSE266350 ATAC-seq GEO GEO: GSE245238, GEO: GSE245246. .. GEO: GSE245247, GEO: GSE245248 RNA-seq GEO GEO: GSE245239, GEO: GSE245245 scRNA-seq GEO GEO: GSE245241 snRNA-seq GEO GEO: GSE245242 scATAC-seq GEO GEO: GSE245243 Amplicon-seq GEO GEO: GSE245244 Experimental Models: Cell Lines KP4, sex: male ATCC RRID: CVCL_1338 PANC-1, sex: male ATCC CRL-1469 MiaPaCa-2, sex: male ATCC CRL-1420 NCI H460, sex: male ATCC HTB-177 NCI H1693, sex: female ATCC CRL-5887 NCI H1793, sex: female ATCC CRL-5896 A549, sex: male ATCC CCL-185 H446, sex: male ATCC HTB-171 H2023, sex: male ATCC CRL-5912 H1568, sex: female ATCC CRL-5876 H661, sex: male ATCC HTB-183 H838, sex: male ATCC CRL-5844 H2286, sex: female ATCC CRL-5938 KP4- inducible BRM oe cells This manuscript N/A KP4 cells overexpressing BRM or BRG1 This manuscript N/A Male mouse lung ATII cells derived organoids with BRG1 knockout This manuscript N/A PANC1 cells overexpressing BRM or BRG1 This manuscript N/A MiaPaCa2 cells overexpressing BRM or BRG1 This manuscript N/A BRM/BRG1 oe cells with NRF2 knock out This manuscript N/A Oligonucleotides On Target Plus siNT pooled siRNA Dharmacon Cat#D-001810-10 On Target Plus siNRF2: pooled siRNA Dharmacon Cat#L-003755-00 (Continued on next page) 20 Cell Reports 43, 114345, June 25, 2024

    TaqMan Assay:

    Article Title: CRISPR activation screens identify the SWI/SNF ATPases as suppressors of ferroptosis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BRM Cell Signaling Cat#11966; RRID: AB_2797783 BRG1 D1Q7F Cell Signaling Cat#49360; RRID:AB_2728743 Beta-Tubulin Cell Signaling Cat#2128; RRID:AB_823664 NRF2 (EP1808Y) Abcam Cat#ab62352; RRID:AB_944418 H3K27me3 Thermofisher Cat#MA511198; RRID:AB_11000749 H3 Cell Signaling Cat#9715; RRID:AB_331563 KEAP1 (P586) Cell Signaling Cat#4678; RRID:AB_10548196 TKT (E7O4M) Cell Signaling Cat#64414; RRID:AB_3097751 GCLC Cell Signaling Cat#48005; RRID: AB_3101780 AKR1B10 (EPR14421) Abcam Cat#ab192865, RRID: AB_3101779 POPDC3 Novus Biologicals Cat#NBP159476; RRID:AB_11034825 BRM for Cut&Run Epicypher Cat#13-2006; RRID:AB_3101778 NRF2 for Cut&Run Active Motif RRID:AB_2793695 SMARCC1 Cell Signaling Cat#11956; RRID:AB_2797776 H3K4me1 Epicypher Cat#13-0057; RRID:AB_3076424 H3K4me3 for Cut&Run Epicypher Cat#13-0041; RRID:AB_3076423 H3K27ac Cell Signaling Cat#8173; RRID:AB_10949503 IgG Epicypher Cat#13-0042; RRID:AB_2923178 Chemicals, Peptides, and Recombinant Proteins Tazemetostat SelleckChem Cat#S7128 Nec1s SelleckChem Cat#S8641 Z-VAD-FMK SelleckChem Cat#S7023 Ferrostatin-1 SelleckChem Cat#S7243 ML210 MIllipore Sigma SML0521 RSL3 Millipore Sigma SML2234 BRM-014 Genentech N/A BRM degrader A947 Genentech N/A Inactive Degrader Control Genentech N/A Imidazole Ketone Erastin SelleckChem Cat#S8877 FIN56 Genentech N/A Critical Commercial Assays Human whole genome SAM CRISPRa pooled lentiviral libraries Sigma Cat#HSAMZEO-1KT TRC3 whole genome lentiviral ORF pool Sigma Cat#ORFPOOLWG – 1SET Chromium Single Cell Gene Expression Reagent Kit v3.1 10x genomics Cat#1000121 Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kit 10x genomics Cat#1000285 Image-IT Lipid peroxidation kit Thermofisher Cat#C10445 Hoescht Thermofisher Cat#33342 CellBrite Steady 650 Biotium Cat#30108 NEBNext 23 PCR Master mix NEB Cat#M0541L Q5 2X Hot start PCR master mix NEB Cat#M0494L QIAquick PCR CleanUp Kit Qiagen Cat#28104 (Continued on next page) Cell Reports 43, 114345, June 25, 2024 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Qiagen MinElute Cleanup Kit Qiagen Cat#28206 Lipofectamine RNAiMAX Transfection Reagent ThermoFisher Cat#13778150 RNeasy extraction kit Qiagen Cat#74034 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7573 Cyquant Direct reagent ThermoFisher Cat#C35011 CUTANA ChIC/CUT&RUN Kit Epicypher Cat#14-1048 CUTANA CUT&RUN Library Prep Kit Epicypher Cat#14-1001 Tagment DNA Enzyme and Buffer Large Kit Illumina Cat20034197 High Sensitivity D1000 Screen Tape and Reagents Agilent Cat#5067-5587 LysoTrackerTM Green DND-26 ThermoFisher Cat#L7526 High Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368813 Taqman Assay for p53 Applied Biosystems Mm01731290_g1 Taqman Assay for SMARCA4 Applied Biosystems Mm01151944_m1 Taqman assay for TUBG Applied Biosystems Mm00506159_m1 Deposited Data All sequencing data Super Series GEO GEO: GSE245249 Cut&Run GEO GEO: GSE245237, GEO: GSE266343,GEO: GSE266350 ATAC-seq GEO GEO: GSE245238, GEO: GSE245246. .. GEO: GSE245247, GEO: GSE245248 RNA-seq GEO GEO: GSE245239, GEO: GSE245245 scRNA-seq GEO GEO: GSE245241 snRNA-seq GEO GEO: GSE245242 scATAC-seq GEO GEO: GSE245243 Amplicon-seq GEO GEO: GSE245244 Experimental Models: Cell Lines KP4, sex: male ATCC RRID: CVCL_1338 PANC-1, sex: male ATCC CRL-1469 MiaPaCa-2, sex: male ATCC CRL-1420 NCI H460, sex: male ATCC HTB-177 NCI H1693, sex: female ATCC CRL-5887 NCI H1793, sex: female ATCC CRL-5896 A549, sex: male ATCC CCL-185 H446, sex: male ATCC HTB-171 H2023, sex: male ATCC CRL-5912 H1568, sex: female ATCC CRL-5876 H661, sex: male ATCC HTB-183 H838, sex: male ATCC CRL-5844 H2286, sex: female ATCC CRL-5938 KP4- inducible BRM oe cells This manuscript N/A KP4 cells overexpressing BRM or BRG1 This manuscript N/A Male mouse lung ATII cells derived organoids with BRG1 knockout This manuscript N/A PANC1 cells overexpressing BRM or BRG1 This manuscript N/A MiaPaCa2 cells overexpressing BRM or BRG1 This manuscript N/A BRM/BRG1 oe cells with NRF2 knock out This manuscript N/A Oligonucleotides On Target Plus siNT pooled siRNA Dharmacon Cat#D-001810-10 On Target Plus siNRF2: pooled siRNA Dharmacon Cat#L-003755-00 (Continued on next page) 20 Cell Reports 43, 114345, June 25, 2024

    Sequencing:

    Article Title: CRISPR activation screens identify the SWI/SNF ATPases as suppressors of ferroptosis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BRM Cell Signaling Cat#11966; RRID: AB_2797783 BRG1 D1Q7F Cell Signaling Cat#49360; RRID:AB_2728743 Beta-Tubulin Cell Signaling Cat#2128; RRID:AB_823664 NRF2 (EP1808Y) Abcam Cat#ab62352; RRID:AB_944418 H3K27me3 Thermofisher Cat#MA511198; RRID:AB_11000749 H3 Cell Signaling Cat#9715; RRID:AB_331563 KEAP1 (P586) Cell Signaling Cat#4678; RRID:AB_10548196 TKT (E7O4M) Cell Signaling Cat#64414; RRID:AB_3097751 GCLC Cell Signaling Cat#48005; RRID: AB_3101780 AKR1B10 (EPR14421) Abcam Cat#ab192865, RRID: AB_3101779 POPDC3 Novus Biologicals Cat#NBP159476; RRID:AB_11034825 BRM for Cut&Run Epicypher Cat#13-2006; RRID:AB_3101778 NRF2 for Cut&Run Active Motif RRID:AB_2793695 SMARCC1 Cell Signaling Cat#11956; RRID:AB_2797776 H3K4me1 Epicypher Cat#13-0057; RRID:AB_3076424 H3K4me3 for Cut&Run Epicypher Cat#13-0041; RRID:AB_3076423 H3K27ac Cell Signaling Cat#8173; RRID:AB_10949503 IgG Epicypher Cat#13-0042; RRID:AB_2923178 Chemicals, Peptides, and Recombinant Proteins Tazemetostat SelleckChem Cat#S7128 Nec1s SelleckChem Cat#S8641 Z-VAD-FMK SelleckChem Cat#S7023 Ferrostatin-1 SelleckChem Cat#S7243 ML210 MIllipore Sigma SML0521 RSL3 Millipore Sigma SML2234 BRM-014 Genentech N/A BRM degrader A947 Genentech N/A Inactive Degrader Control Genentech N/A Imidazole Ketone Erastin SelleckChem Cat#S8877 FIN56 Genentech N/A Critical Commercial Assays Human whole genome SAM CRISPRa pooled lentiviral libraries Sigma Cat#HSAMZEO-1KT TRC3 whole genome lentiviral ORF pool Sigma Cat#ORFPOOLWG – 1SET Chromium Single Cell Gene Expression Reagent Kit v3.1 10x genomics Cat#1000121 Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kit 10x genomics Cat#1000285 Image-IT Lipid peroxidation kit Thermofisher Cat#C10445 Hoescht Thermofisher Cat#33342 CellBrite Steady 650 Biotium Cat#30108 NEBNext 23 PCR Master mix NEB Cat#M0541L Q5 2X Hot start PCR master mix NEB Cat#M0494L QIAquick PCR CleanUp Kit Qiagen Cat#28104 (Continued on next page) Cell Reports 43, 114345, June 25, 2024 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Qiagen MinElute Cleanup Kit Qiagen Cat#28206 Lipofectamine RNAiMAX Transfection Reagent ThermoFisher Cat#13778150 RNeasy extraction kit Qiagen Cat#74034 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat#G7573 Cyquant Direct reagent ThermoFisher Cat#C35011 CUTANA ChIC/CUT&RUN Kit Epicypher Cat#14-1048 CUTANA CUT&RUN Library Prep Kit Epicypher Cat#14-1001 Tagment DNA Enzyme and Buffer Large Kit Illumina Cat20034197 High Sensitivity D1000 Screen Tape and Reagents Agilent Cat#5067-5587 LysoTrackerTM Green DND-26 ThermoFisher Cat#L7526 High Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368813 Taqman Assay for p53 Applied Biosystems Mm01731290_g1 Taqman Assay for SMARCA4 Applied Biosystems Mm01151944_m1 Taqman assay for TUBG Applied Biosystems Mm00506159_m1 Deposited Data All sequencing data Super Series GEO GEO: GSE245249 Cut&Run GEO GEO: GSE245237, GEO: GSE266343,GEO: GSE266350 ATAC-seq GEO GEO: GSE245238, GEO: GSE245246. .. GEO: GSE245247, GEO: GSE245248 RNA-seq GEO GEO: GSE245239, GEO: GSE245245 scRNA-seq GEO GEO: GSE245241 snRNA-seq GEO GEO: GSE245242 scATAC-seq GEO GEO: GSE245243 Amplicon-seq GEO GEO: GSE245244 Experimental Models: Cell Lines KP4, sex: male ATCC RRID: CVCL_1338 PANC-1, sex: male ATCC CRL-1469 MiaPaCa-2, sex: male ATCC CRL-1420 NCI H460, sex: male ATCC HTB-177 NCI H1693, sex: female ATCC CRL-5887 NCI H1793, sex: female ATCC CRL-5896 A549, sex: male ATCC CCL-185 H446, sex: male ATCC HTB-171 H2023, sex: male ATCC CRL-5912 H1568, sex: female ATCC CRL-5876 H661, sex: male ATCC HTB-183 H838, sex: male ATCC CRL-5844 H2286, sex: female ATCC CRL-5938 KP4- inducible BRM oe cells This manuscript N/A KP4 cells overexpressing BRM or BRG1 This manuscript N/A Male mouse lung ATII cells derived organoids with BRG1 knockout This manuscript N/A PANC1 cells overexpressing BRM or BRG1 This manuscript N/A MiaPaCa2 cells overexpressing BRM or BRG1 This manuscript N/A BRM/BRG1 oe cells with NRF2 knock out This manuscript N/A Oligonucleotides On Target Plus siNT pooled siRNA Dharmacon Cat#D-001810-10 On Target Plus siNRF2: pooled siRNA Dharmacon Cat#L-003755-00 (Continued on next page) 20 Cell Reports 43, 114345, June 25, 2024

    Chromatin Immunoprecipitation:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

    DNA Purification:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

    Imaging:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

    Conjugation Assay:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

    Gas Chromatography-Mass Spectrometry:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

    Polymerase Chain Reaction:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

    Purification:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

    Sample Prep:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

    DNA Sequencing:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5

    RNA Sequencing:

    Article Title: Cystine import and oxidative catabolism fuel vascular growth and repair via nutrient-responsive histone acetylation.
    Article Snippet: Compound Sakura Cat#4583 TRIS Roth Cat#AE15.3 Triton X-100 Sigma-Aldrich Cat#9002-93-1 Trizol QIAGEN Cat#79306 trypsin Biochrom Cat#L2103-20G Trypsin-Inhibitor Applichem Cat#A1441,0250 (Continued on next page) e4 Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER TURBO DNAseI Thermo Fisher Scientific Cat#AM223 U-13C16-palmitic acid Cambridge Isotope Laboratories Cat#CLM-309-PK Critical commercial assays Chromatin IP DNA Purification Kit Active Motif Cat#58002 Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10337 DyLight 405 Conjugation kit Novus Biologicals Cat#ab201798 EZ:faast GC-MS Physiological, Amino Acid Analysis Kit Phenomenex Cat#KG0-7166 FITC Annexin V Apoptosis Detection Kit I BD Pharmingen Cat#556547 Illumina Tagment DNA TDE1 Enzyme and Buffer Large Kit Illumina Cat20034198 MycoStrip – Mycoplasma Detection Kit Invivogen Cat#rep-mys-10 QIAquick PCR Purification Kit QIAGEN Cat#28104 SMARTer Stranded Total RNA Sample Prep Kit HI Mammalian Takara Bio Cat#634873 ThruPLEX DNA-Seq Kit Takara Bio Cat#R400674 VAHTS Universal Stranded mRNA-seq - V6 Vazyme Cat#NR604 Zymo ChIP DNA Clean and concentrator Kit Zymo Research Cat#D5205 Deposited data RNA-seq data This paper GEO: GSE305819 ATAC-seq data This paper GEO: GSE305823; GSE305822 Ribotag-seq data This paper GEO: GSE305827 CUT&RUN/Act-seq data This paper GEO: GSE305820; GSE305824; and GSE305826 Proteomics data This paper PRIDE: PXD068385 Raw data This paper Data S1 Experimental models: Cell lines Human: umbilical vein endothelial cells (HUVEC) Lonza Promocell Cat#C2519A; #C2517; #00191027; Cat#C12203; #C12200; #C12205 Human: 293 American Type Culture Collection (ATCC) Cat#CRL-1573 Lenti-XTM HEK 293T Takara Cat#632180 Experimental models: Organisms/strains SLC7A11 fl/fl xPdgfb-CreERT2 Shanghai model Organisms And The Jackson Laboratory C57BL/6Smoc-Slc7a11em1(flox)Smoc: Cat#NM-CKO-2117850B6.Cg-Tg(Pdgfrbcre/ERT2)6096Rha/J: Cat#029684 PdgfbCreERT2 The Jackson Laboratory B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J; Strain#029684 CSE fl/fl xPdgfb-CreERT2 This paper N/A CSE fl/fl xCdh5-CreERT2 https://doi.org/10.1161/CIRCULATIONAHA. .. 118.034757 N/A FUCCI Riken Bioresource research center B6.Cg-Tg(FUCCIS/G2/M)#504BsiTg (FUCCIG1)#596Bsi; Cat#RBRC02892 WT mice (C57BL/6JRj) Janvier Labs C57BL/6JRj Mouse - Janvier Labs Recombinant DNA pCDH-CMV-3xFlag–human-ATF4-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-CSE-copGFP Synthetized by Genomeditech, Shanghai, China N/A pCDH-CMV-3xFlag–human-SLC7A11copGFP Synthetized by Genomeditech, Shanghai, China N/A (Continued on next page) Cell Metabolism 38, 1–20.e1–e20, January 6, 2026 e5



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    Illumina Inc buffer large kit illumina
    Buffer Large Kit Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    buffer large kit illumina - by Bioz Stars, 2026-09
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    Illumina Inc tde1 enzyme
    Tde1 Enzyme, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc tde1 tagment dna enzyme
    Tde1 Tagment Dna Enzyme, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tde1 tagment dna enzyme - by Bioz Stars, 2026-09
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    Illumina Inc illumina tagment dna enzyme
    Illumina Tagment Dna Enzyme, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc buffer large kit
    Buffer Large Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc illumina tn5 enzyme
    Illumina Tn5 Enzyme, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    illumina tn5 enzyme - by Bioz Stars, 2026-09
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    Illumina Inc tagment dna enzyme 1
    Tagment Dna Enzyme 1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc transposition mix
    Transposition Mix, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc tagment dna enzyme
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